stmn2 mouse tagged orf (OriGene)
Structured Review

Stmn2 Mouse Tagged Orf, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stmn2+mouse+tagged+orf/bio_rxiv__64898__2026__04__02__715646-35-5-10?v=OriGene
Average 94 stars, based on 1 article reviews
Images
1) Product Images from "Stathmin-2 Mediates Paracrine Hormone Regulation of Glucagon Through Lysosomal Trafficking in αTC1-6 cells"
Article Title: Stathmin-2 Mediates Paracrine Hormone Regulation of Glucagon Through Lysosomal Trafficking in αTC1-6 cells
Journal: bioRxiv
doi: 10.64898/2026.04.02.715646
Figure Legend Snippet: A) Images show co-localized immunofluorescence signals for glucagon and Stmn2 (top row), glucagon and the lysosomal marker LAMP1 (middle row) and glucagon and the secretory granule membrane docking protein syntaxin-1A (bottom row). Insets show magnified areas outlined in green. Yellow arrowheads indicate the spatial localization of immunofluorescence signals in peripheral and intracellular regions. B) Plot profile analyses of colocalized immunofluorescence signals in the intracellular or peripheral regions in response to vehicle (open circle), 1 nM insulin+25 μM GABA (open squares), or 400 nM SST (x). Bars represent the averaged colocalized fluorescence intensities ± SEM (n=3) and symbols represent measurements from individual cells. Comparisons were made between and within cellular regions using a two-way ANOVA, followed by a post-hoc test. ***p<0.001, ****p<0.0001.
Techniques Used: Immunofluorescence, Marker, Membrane, Fluorescence
Figure Legend Snippet: Transfected cells were immunostained using primary antibodies against Stmn2 (green) and glucagon (red). a) Cells transfected with GFP alone show co-localized signals mainly at the cell periphery (yellow arrowheads). b) In Stmn2-KD cells, there is diminished Stmn2 immunofluorescence with glucagon localized largely to the periphery (yellow arrowheads). c) After Stmn2 OE, there is increased Stmn2 fluorescence intensity colocalizing strongly with glucagon in the intracellular region (yellow arrowheads). d) Changes in integrated Stmn2 fluorescence intensity in the Stmn2-KD and OE compared to the control (GFP-alone), normalized to endogenous Stmn2. Values were expressed as average integrated fluorescence intensity ±SEM (n=6) and compared among groups using a one-way ANOVA, followed by a post-hoc test. **p<0.01, ****p<0.0001. e) Glucagon distribution under control conditions (dark grey bars), and in response to Stmn2-KD (black bars) or OE (light grey bars) with values expressed as average glucagon fluorescence intensity ±SEM (n=6), compared among groups and cellular regions using a two-way ANOVA, followed by a post-hoc test. **p<0.01, ****p<0.0001.
Techniques Used: Transfection, Immunofluorescence, Fluorescence, Control
Figure Legend Snippet: Transfected cells were treated with 1 nM insulin for 24 hours, then immunostained using primary antibodies against Stmn2 and glucagon. a) Cells transfected with GFP vector and treated with 1 nM insulin showed Stmn2 (green) and glucagon (red) redistributing intracellularly. b) The Stmn2-KD model diminished Stmn2 fluorescence and resulted in glucagon in the periphery, even after 1 nM insulin treatment. c) The Stmn2-OE model increased Stmn2 fluorescence intensity that colocalized strongly with glucagon in the intracellular region after 1 nM insulin treatment. d) Quantification of glucagon distribution in response to Stmn2-KD and OE with values expressed as average glucagon fluorescence intensity ±SEM (n=6), compared among groups and cellular regions using a two-way ANOVA, followed by a post-hoc test. ****p<0.0001.
Techniques Used: Transfection, Plasmid Preparation, Fluorescence
Figure Legend Snippet: Transfected cells were treated with 400 nM somatostatin for 24 hours. Cells were then immunostained using primary antibodies against Stmn2 (green) and glucagon (red). a) Cells transfected with GFP vector and treated with 400 nM somatostatin resulted in Stmn2 and glucagon distributing mostly in the intracellular region (yellow arrowheads). b) The Stmn2-KD model diminished Stmn2 fluorescence intensity, and glucagon was present in the periphery after somatostatin treatment. c) The Stmn2-OE model increased Stmn2 fluorescence intensity while colocalizing strongly with glucagon in the intracellular region after somatostatin treatment. d) Glucagon distribution in response to Stmn2-KD (red bars) and OE (green bars) with values expressed as average glucagon fluorescence intensity ±SEM (n=6) and compared among groups and cellular regions using a two-way ANOVA, followed by a post-hoc test. ****p<0.0001.
Techniques Used: Transfection, Plasmid Preparation, Fluorescence
Figure Legend Snippet: Cells (n=3) were co-transfected with LAMP1-RFP and GFP vector or Stmn2-GFP, or with scrambled siRNA or siRNA against stathmin-2 (Stmn2-KD) and live images were captured 24 hours post-transfection. a) LAMP1-RFP is present in the cell periphery and the intracellular region (yellow arrowheads) after co-transfection with GFP alone as a negative control (upper panel). After co-transfection with Stmn2-GFP, (lower panel), LAMP1-RFP appears exclusively in the intracellular region (yellow arrowheads). b) LAMP1-RFP is present in the cell periphery and the intracellular region (yellow arrowheads) after co-transfection with scrambled siRNA sequences as a negative control for Stmn2-KD (upper panel). Transfection with Stmn2 siRNAs (KD) resulted in LAMP1-RFP exclusively in the cell periphery (yellow arrowheads) (lower panel). c) Transcription factor EB (TFEB) is not translocated to the nucleus upon overexpression of Stmn2-GFP. Immunofluorescence images of fixed cells show the subcellular distribution of TFEB, GFP alone (upper panel), Stmn2-GFP (lower panel) and cell nuclei (DAPI). Box plots show the ratio of nuclear to cytoplasmic TFEB immunofluorescence. Values were expressed as the average nuclear/cytoplasmic TFEB intensity ±SEM (n=4). Data points in colour represent the average ratio of 8-16 cells per coverslip; black points represent ratios from individual cells. d) Stmn2 regulates the lysosomal transport protein Arl8. Immunofluorescence images of fixed cells show the presence of Arl8 (orange) in cells transfected with scrambled siRNA or siRNA against Stmn2. Quantification of fluorescence (left) shows that knockdown of Stmn2 (Stmn2-KD) significantly increased the fluorescence intensity of Arl8. Values are means ±SEM (n=6). Data points in colour represent the average fluorescence intensities in 8-16 cells per coverslip; black points represent values from individual cells.
Techniques Used: Transfection, Plasmid Preparation, Cotransfection, Negative Control, Over Expression, Immunofluorescence, Fluorescence, Knockdown
Figure Legend Snippet: Live images of αTC1-6 cells transfected with LAMP1-RFP (n=3) were captured in the same cell before, during, and five minutes after treatment with 1 nM insulin. a) LAMP1-RFP redistributed intracellularly (yellow arrowheads) in response to 1 nM insulin treatment in control cells transfected with scrambled siRNA. b) LAMP1-RFP distribution was not changed after 1 nM insulin treatment after siRNA-mediated knockdown of Stmn2 (Stmn2-KD) (yellow arrowheads). Graphs show the distribution of LAMP1-RFP fluorescence intensities in the intracellular and peripheral regions before (pre-INS) and after (post-INS) treatment with 1 nM insulin. Values are means ±SEM (n=3). * p<0.05
Techniques Used: Transfection, Control, Knockdown, Fluorescence
Figure Legend Snippet: αTC1-6 cells (n=3) were transfected overnight. Live images were captured in the same cell before, during, and five minutes after treatment with 400 nM somatostatin. a) LAMP1-RFP redistributed intracellularly (yellow arrowheads) in response to 400 nM somatostatin treatment in control cells transfected with scrambled siRNA. b) LAMP1-RFP distribution was not changed after 400 nM somatostatin treatment after siRNA-mediated knockdown of Stmn2 (Stmn2-KD) (yellow arrowheads). Graphs show means ±SEM (n=3). * p<0.05
Techniques Used: Transfection, Control, Knockdown